Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC TAE-1 25mg | 1414469-59-2 | 25 MG
TAE-1 is a small-molecule inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) that also inhibits amyloid-β fibril formation and aggregation. It is used in biochemical and cellular research into Alzheimer's disease, cholinesterase function, and amyloid-β aggregation.
- Potent inhibitor of AChE and BuChE.
- Inhibits amyloid-β fibril formation and aggregation.
- Molecular formula C39H51I3N6O9 and molecular weight 1128.57.
- Typical supplied purity about 96.17%.
- Available in small laboratory pack sizes (for example, 25 mg) for research use.
INNOVAPREP LLC Elution Fluid Can, Tris-buffered solution, Water >99.9%, Tween 20 0.075%, Carbon Dioxide propellant, 125 psi pressure, 50 extractions per can, Non-flammable gas
The InnovaPrep HC08001 Elution Fluid Can is a specially formulated Tris-buffered solution designed for efficient recovery of particles using the InnovaPrep Concentrating Pipette systems. This carbonated elution fluid employs patented Wet Foam Elution™ technology for high recovery rates of microorganisms and particles, maintaining their integrity for various analytical applications. The pressurized can format enhances laboratory workflows with its ready-to-use capability, facilitating rapid and reliable sample concentration.
- Optimized particle recovery through Wet Foam Elution™ technology
- Preserves integrity for downstream compatibility
- Supports up to 50 extractions per can for high throughput
- Pressurized can ensures safe and immediate use
Bioworld PIPES BUFFER 1M PH 6.0 100ML
liquid, 1.0m, ph 6Synonym(s): 1,4-Piperazinediethanesulfonic acidMolecular Formula: C8H18N2O6S2CAS Number: 5625-37-6Molecular Weight: 302.37
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 500g
Linear Formula NH2C(CH2OH)3, ≥99.9% (titration), Molecular Weight 121.14, bp 219-220 °C/10 mmHg(lit.), mp 167-172 °C (lit.), Primary Standard and Buffer, crystalline, Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
ATHENA ES HBS w/Phenol Red, 500 ml
Hepes Buffered Saline is available with or without Phenol Red. It is an isotonic saline buffered with Hepes, for use in rinsing cell monolayers prior to cell dissociation using PETTM or washing harvested cells.
Apexbio Technology LLC Annexin V-Cy5/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Apexbio Technology LLC Protease Inhibitor Cocktail (100X in DMSO EDTA plus) 100x(1ml A 1ml B)
This product is a protease inhibitor cocktail (100X in DMSO EDTA plus) specifically designed for use during cell lysis and protein extraction processes It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases Also included is a separate 0 5 M EDTA solution which effectively inhibits metalloproteases The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO capable of efficiently inhibiting serine proteases cysteine proteases aspartic proteases and aminopeptidases commonly present in cell lysates EDTA is provided for inhibition of metalloproteases If immobilized metal affinity chromatography (IMAC) is involved for protein purification or if two-dimensional (2D) gel electrophoresis analysis is planned EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification Specification 1 Set equals 1X (1 mL A 1 mL B) 10 Sets equals 10X (1 mL A 1 mL B) and so on